of medicinal plants and traditional health systems in solving the health care problems of the world exist from time immemorial. Man explored his immediate natural surroundings for eternal health and longevity and for remedies to relieve pain and discomfort. Wound healing is the process of repair that follows injury to the skin and other soft tissues. The barks of four Ficus species, referred to as „Nalpamaram? in Southern part of India, namely, F. benghalensis, F. racemosa, F. religiosa, and F. microcarpa, are considered to be very effective as anti-septic, antioxidant, astringent, and anti-inflammatory agents. In the present study we have developed a method for standardization of the formulation „NalpamaradiChoorna?, containing the barks of these four species, using HPTLC, a modern analytical technique. The herbal formulation was standardized using Caffeic acid and Alpha lupeol as marker compounds for the first time. The method is found to be simple, precise, reproducible and linear in the range of 100-800 ng for Caffeic acid and 50-200 ng for Lupeol. Keywords:Ficus sp., Nalpamaradichoorna, Standardization, HPTLC, Caffeic acid, alpha lupeol . T