Biology or Biological science is a branch of science that studies living organisms. The main aim of the present study is to achieve a comparative analysis as to which method is effective in Biology teaching the traditional method i.e. using Blackboard or multimedia method i.e. using PowerPoint Slides, Printed transparencies on OHP, etc. In the present research study the control group was taught certain topics of biology using the traditional chalk and blackboard, whereas, the same topics of biology were taught to the experimental students by using multimedia PowerPoint presentation. In the pretest of the control group, the value of mean, median and mode are 6.56, 7.5 and 9 respectively. In the pretest of the experimental group, the value of mean, median and mode are 6.26, 5.5 and 4 respectively. In the posttest of the control group, the value of mean, median and mode are 16.5, 16.5 and 14 respectively. In the posttest of the experimental group, the value of mean, median and mode are 23, 23 and 21 respectively. The unpaired “t” test analysis showed that there was no significant difference in the pretest achievement scores of experimental and control group in certain topics in biology. While significant difference in the posttest achievement scores of experimental and control group in certain topics in biology. Researcher has observed mark improvement in achievement test scores in experimental group student’s rather than control group students. Multimedia teaching and learning has contributed positively and enhancement of learning is being observed in the experimental group students.